Filter low-expressed genes, at least

meet the number of reads of more than

Genes with a significance test p-value less than

and the absolute value of the fold change greater than

will be marked as significantly different.

The top

in ascending p-value order of the

regulation will be plotted as a heatmap.

Analysis type:

Have reference genome annotation:

• yes no

Choose a reference genome: Zebrafish Human Mouse Rat Rhesus Fly Worm(C.elegans) Arabidopsis Yeast Chicken Chimp Pig

Go enrichment classification display:

Transform the y-axis data:

Choose a reference genome:

Removed free interactions:

• yes no

Choose a layout:

and edge style:

and edge colour:

Node colour:

and node alpha:

Add node text:

• yes no

Dege greater than

and repel:

• yes no

and size:

Standardized settings:

• yes no

Method distance settings:

Confidence ellipse:

• no yes

• no yes

The type of ellipse

The level at which to draw an ellipse

Bar type:

• Dar graph of mean draw bar charts

Subgroup name:

Group mapping colour:

Add err bar:

• yes no

Add all Significance markers:

• no compare First yes

• 1: If you want to personalize the add error line, select No and then add it manually inside the detail plot

The p-value is replaced by an asterisk:

• yes no

Statistical method

x-axis data column name

and x-axis data type

y-axis data column name

and y-axis data type

The final rendered point-sized column is named

The column finally rendered as point color is named

Use newly specified parameters to determine up-down categories :

• no yes

Up and down grouping category column namesUp and down grouping category column names

Genes with a significance test p-value less than (padj)

and the absolute value of the fold change greater than (log2FoldChange)

Show elements:

• no yes

. Elements sep:

Show percentage:

• yes no

. Persentage text digits:

garden edge color:

alpha:

size:

linetype:

Set name color:

size:

text color:

size:

Choose different plot methods:

• ggplot2(complex and detailed) pheatmap(simple and Efficient)

Add sample group:

• no yes

Show group legend:

• yes no

Show number:

• no yes

Picture title:

Text angle: