Bioproject ID: PRJNA545230

bioproject id PRJNA545230      to NCBI
key word baseline;GERM-LAYER FORMATION;ONE-EYED-PINHEAD;BLIND CAVEFISH;PRECHORDAL PLATE;AXIAL MESENDODERM;AST
experiment type baseline
publication Torres-Paz, J. , J. Leclercq , and S Rétaux. "Maternally-regulated gastrulation as a source of variation contributing to cavefish forebrain evolution." eLife Sciences 8(2019).
description RNA sequencing of 2-cells eggs of Astyanax mexicanus (cavefish, surface fish and hybrids coming from reciprocal crosses) to compare maternally deposit RNAs and link them to the developmental variations observed between the two morphotypes.
abstract Sequential developmental events, starting from the moment of fertilization, are crucial for the acquisition of animal body plan. Subtle modifications in such early events are likely to have major impacts in later morphogenesis, bringing along morphological diversification. Here, comparing the blind cave and the surface morphotypes of Astyanax mexicanus fish, we found heterochronies during gastrulation that produce organizer and axial mesoderm tissues with different properties (including differences in the expression of dkk1b) that may have contributed to cavefish brain evolution. These variations observed during gastrulation depend fully on maternal factors. The developmental evolution of retinal morphogenesis and hypothalamic patterning are among those traits that retained significant maternal influence at larval stages. Transcriptomic analysis of fertilized eggs from both morphotypes and reciprocal F-1 hybrids showed a strong and specific maternal signature. Our work strongly suggests that maternal effect genes and developmental heterochronies that occur during gastrulation have impacted morphological brain change during cavefish evolution.

Sample Information

sample id sample name tissue strain treatment description
1. SRR9141190 Hybrid CF 2-cells egg nan untreatment RNA-Seq of Astyanax mexicanus hybrid cavefish: 2-cells stage Replicate 2
2. SRR9141191 Hybrid CF 2-cells egg nan untreatment RNA-Seq of Astyanax mexicanus hybrid cavefish: 2-cells stage Replicate 3
3. SRR9141192 SF 2-cells egg nan untreatment RNA-Seq of Astyanax mexicanus surface fish: 2-cells stage Replicate 3
4. SRR9141193 CF 2-cells egg nan untreatment RNA-Seq of Astyanax mexicanus cavefish: 2-cells stage Replicate 1
5. SRR9141194 SF 2-cells egg nan untreatment RNA-Seq of Astyanax mexicanus surface fish: 2-cells stage Replicate 1
6. SRR9141195 SF 2-cells egg nan untreatment RNA-Seq of Astyanax mexicanus surface fish: 2-cells stage Replicate 2
7. SRR9141196 Hybrid SF 2-cells egg nan untreatment RNA-Seq of Astyanax mexicanus hybrid surface fish: 2-cells stage Replicate 1
8. SRR9141197 Hybrid SF 2-cells egg nan untreatment RNA-Seq of Astyanax mexicanus hybrid surface fish: 2-cells stage Replicate 2
9. SRR9141198 CF 2-cells egg nan untreatment RNA-Seq of Astyanax mexicanus cavefish: 2-cells stage Replicate 2
10. SRR9141199 CF 2-cells egg nan untreatment RNA-Seq of Astyanax mexicanus cavefish: 2-cells stage Replicate 3
11. SRR9141200 Hybrid SF 2-cells egg nan untreatment RNA-Seq of Astyanax mexicanus hybrid surface fish: 2-cells stage Replicate 3
12. SRR9141201 Hybrid CF 2-cells egg nan untreatment RNA-Seq of Astyanax mexicanus hybrid cavefish: 2-cells stage Replicate 1