Bioproject ID: PRJNA429670

bioproject id PRJNA429670      to NCBI
key word Chemical; diet; Sodium butyrate; Ctenopharyngodon idella; feed additives; intestinal immune system; organic acids; transcriptome; PACIFIC WHITE SHRIMP; GROWTH-PERFORMANCE; DIETARY SUPPLEMENTATION; NILE TILAPIA; TRANSPORTER PEPT1; SKELETAL-MUSCLE; PROTECTED FORMS; UP-REGULATION; KAPPA-B; IDENTIFICATION
experiment type chemical
publication Tang X et al., "Effects of sodium butyrate stimulation on immune‐related mRNA‐miRNA network in intestine of grass carp", Aquaculture Research, 2021; 52:309–322
description Sodium butyrate is one of the most popular feed additives in animal husbandry while less used in fish feeding. However, the supplementation of sodium butyrate is increasing recently in aquaculture. The goal of the present study was to unveil the intestinal mRNA and microRNA (miRNA) response to feed with sodium butyrate in an important cultured fish species, grass carp (Ctenopharyngodon idella).
abstract Sodium butyrate is one of the most popular feed additives in animal husbandry. In recent years, sodium butyrate has been increasingly used as supplement in aquaculture. The present study is to investigate the intestinal mRNA and microRNA response to diet with sodium butyrate in grass carp (Ctenopharyngodon idella), an important aquaculture species in China. mRNA and microRNA profiles of intestine of grass carp fed with diet contained 0, 1.0, 2.5, 5.0, 7.5 and 10.0 g/kg sodium butyrate were obtained by RNA‐seq using Illumina Hiseq 2,500 platform. The feeding trial was performed using 18 individuals of 1‐year‐old grass carp (n = 3 for each group) and lasted for 40 days in tanks in laboratory. A total of 349,860,852 sequence reads were generated from six intestinal libraries. Functional analysis of differentially expressed genes showed that genes participated in immune pathways tend to be activated by sodium butyrate supplementation. A total of 700 microRNAs were obtained, including 275 conserved microRNAs and 425 novel microRNAs which are potentially involved in regulating 14,300 genes. Spearman's correlation analysis identified 18 pairs of microRNA‐mRNA associated with immune pathways (p < .01 and R<−0.5). The potential genes targeted by microRNAs include CXCL12, AKT1S1, Cab39 and MHCII which are important genes associated with intestinal immune pathways. To our knowledge, this is the first integrated profiling of both mRNA and microRNA in intestine with supplementation of sodium butyrate in grass carp. The present results suggest that sodium butyrate affects intestinal immune system by regulating microRNA‐mRNA interaction in fish.

Sample Information

sample id sample name tissue strain treatment description
1. SRR6475468 1.0_SB intestine nan fed with 1.0 g/kg sodium butyrate fed with 1.0 g/kg sodium butyrate
2. SRR6475469 Control intestine nan control control
3. SRR6475470 5.0_SB intestine nan fed with 5.0 g/kg sodium butyrate fed with 5.0 g/kg sodium butyrate
4. SRR6475471 2.5_SB intestine nan fed with 2.5 g/kg sodium butyrate fed with 2.5 g/kg sodium butyrate
5. SRR6475472 7.5_SB intestine nan fed with 7.5 g/kg sodium butyrate fed with 7.5 g/kg sodium butyrate
6. SRR6475475 10.0_SB intestine nan fed with 10.0 g/kg sodium butyrate fed with 10.0 g/kg sodium butyrate