| bioproject id |
PRJNA476389
to NCBI
|
| key word |
Challenge; GCRV; CIK; Aquareovirus; NS31; Heat-shock response; HSP70; CELLULAR HEAT-SHOCK-PROTEIN-70; VIRUS; HSP70; ACTIVATION; POLYMERASE; CHAPERONES; ADENOVIRUS; PROMOTERS; MECHANISM |
| experiment type |
challenge |
| publication |
Yu F etal., "Aquareovirus NS31 protein serves as a specific inducer for host heat shock 70-kDa protein", Journal of General Virology, 2020; 101:145-155 |
| description |
To understand the effects of Grass carp reovirus （GCRV） infection on transcriptions of grass carp genes, RNA-seq of CIK cells infected with Grass carp reovirus JX01 or overexpressed with viral encoding protein NS31 was carried out. |
| abstract |
Elevation of heat-shock protein expression, known as cellular heat-shock responses, occurs during infection of many viruses. which is involved in viral replication through various mechanisms. Herein, transcriptome analysis revealed that over-expression of non-structural protein NS31 of grass carp reovirus (GCRV) in grass carp Ctenopharyngodon idellus kidney (CIK) cells specifically induced expression of heat-shock response (HSR) genes HSP30 and HSP70. We further found that, among the HSR genes, only HSP70 protein were shown to be efficiently induced in fish cells following NS31 over-expression or GCRV infection. Employing a luciferase assay, we were able to show that the promoter of the HSP70 gene can be specifically activated by NS31. In addition, over-expressing HSP70 in grass carp CIK cells resulted in enhanced replication efficiency of GCRV, and an inhibitor for HSP70 resulted in the inhibition of GCRV replication, indicating that HSP70 should serve as a pro-viral factor. We also found that NS31 could activate HSP70 expression in cells of other vertebrate animals. Similar inducing effect on HSP70 expression was demonstrated for NS31-homologue proteins of other aquareoviruses including American grass carp reovirus (AGCRV) and GRCV (green river chinook virus). All these results indicated NS31 proteins in the Aquareovirus genus should play a key role for up-regulating specific HSP70 gene during viral replication. |
Gene
RNA