Bioproject ID: PRJNA481437

bioproject id PRJNA481437      to NCBI
key word Chemical; luteinizing hormone-releasing hormone agonist; LHRH-A; Grass carp; LHRH-A; Lipid metabolism; Gene ontology; KEGG pathway
experiment type chemical
publication Zhong H et al., "Transcriptomic evidence of luteinizing hormone-releasing hormone agonist (LHRH-A) regulation on lipid metabolism in grass carp (Ctenopharyngodon idella)", Genomics, inpress.
description After 7 hours post injection with 0.05 µg/g LHRH-A or saline, the liver of grass carp (1 year old) was collected and sequenced using Illumina Hiseq 4000.
abstract In this study, RNA sequencing was used to identify the hepatic gene expression profile in grass carp associated with luteinizing hormone-releasing hormone agonist (LHRH-A) treatment. A total of 93,912,172 reads were generated by HiSeq 4000 sequencing platform. After filtering, 83,450,860 clean reads were mapped to the reference genome. By calculating the FPKM of genes, 1475 differentially expressed genes were identified. PPAR signaling pathway was enriched with upregulated genes in LHRH-A injection group showing the regulation of the lipid metabolism by LHRH-A. The expression of eight key genes in PPAR signaling pathway was confirmed by qPCR and the results suggested that ACSL4A, ACSL4B, ANGPTL4, LPL, RXRBA and SLC27A1B were significantly stimulated by LHRH-A injection. This investigation provides the evidence that LHRH-A could play a role in lipid metabolism.

Sample Information

sample id sample name tissue strain treatment description
1. SRR7533557 LHRHA-liver liver Changsha injected with luteinizing hormone-releasing hormone agonist (LHRH-A) Liver of grass carp treated with LHRHA
2. SRR7533558 Control_liver liver Changsha untreatment Liver of grass carp (Control)