Bioproject ID: PRJNA530387

bioproject id PRJNA530387      to NCBI
key word Vaccine, VHSV, IPNV, antibodies, B cell repertoire, heterologous immunization, public response, fish immunology, RepSeq, comparative immunology
experiment type vaccine
publication Navelsaker, S. , et al. "Sequential Immunization With Heterologous Viruses Does Not Result in Attrition of the B Cell Memory in Rainbow Trout." Frontiers in Immunology (2019):2687.
description Ig repertoire of Fish vaccinated against VHSV and infected by IPNV
abstract Long-term immunity is of great importance for protection against pathogens and has been extensively studied in mammals. Successive heterologous infections can affect the maintenance of immune memory, inducing attrition of T memory cells and diminishing B cell mediated protection. In fish, the basis of immune memory and the mechanisms of immunization to heterologous pathogens remain poorly understood. We sequentially immunized isogenic rainbow trout with two immunologically distinct viruses, VHSV and IPNV, either with one virus only or in combination, and analyzed the antibody responses and repertoires. Neutralizing antibodies and ELISPOT did not reveal an effect of heterologous immunization. Using a consensus read sequencing approach that incorporates unique barcodes to each cDNA molecule, we focused on the diversity expressed by selected responding VH/C combinations. We identified both public and private responses against VHSV and/or IPNV in all groups of fish. In fish immunized with two viruses, we registered no significant reduction in the persistence of the response toward the primary immunization. Similarly, the response to the second immunization was not affected by a prior vaccination to the other virus. Our data suggest that heterologous immunization does not enforce attrition of pre-existing antibody producing cells, which may impair the protection afforded by multiple successive vaccinations. These observations are potentially important to improve vaccination strategies practiced in aquaculture.

Sample Information

sample id sample name tissue strain treatment description
1. SRR8835976 5B3 spleen Isogenic line B57 Fish vaccinated agains VHSV and infected by IPNV nan
2. SRR8835982 7.3 spleen Isogenic line B57 Fish infected by IPNV nan
3. SRR8835975 5B4 spleen Isogenic line B57 Fish vaccinated agains VHSV and infected by IPNV nan
4. SRR8835979 7.2 spleen Isogenic line B57 Fish infected by IPNV nan
5. SRR8835977 5B1 spleen Isogenic line B57 Fish vaccinated agains VHSV and infected by IPNV nan
6. SRR8835981 7.4 spleen Isogenic line B57 Fish infected by IPNV nan
7. SRR8835971 5B8 spleen Isogenic line B57 Fish vaccinated agains VHSV, infected by IPNV, then boosted by VHSV nan
8. SRR8835972 5B7 spleen Isogenic line B57 Fish vaccinated agains VHSV, infected by IPNV, then boosted by VHSV nan
9. SRR8835974 5B5 spleen Isogenic line B57 Fish vaccinated agains VHSV, infected by IPNV, then boosted by VHSV nan
10. SRR8835973 5B6 spleen Isogenic line B57 Fish vaccinated agains VHSV, infected by IPNV, then boosted by VHSV nan
11. SRR8835980 7.1 spleen Isogenic line B57 Fish infected by IPNV nan
12. SRR8835978 5A3 spleen Isogenic line B57 Fish vaccinated agains VHSV and infected by IPNV nan