Gene
RNA
| bioproject id | PRJNA295899 to NCBI |
| key word | Challenge, Yersinia ruckeri cells, PBS |
| experiment type | challenge |
| publication | Michela, F. , et al. "Induced expression of cathelicidins in trout (Oncorhynchus mykiss) challenged with four different bacterial pathogens." Journal of Peptide ence 24(2018):e3089. |
| description | Whole transcriptome of fishes injected with inactivated Yersinia ruckeri cells or PBS. |
| abstract | Cathelicidins are an important family of antimicrobial peptide effectors of innate immunity in vertebrates. Two members of this group, CATH‐1 and CATH‐2, have been identified and characterized in teleosts (ray‐finned fish). In this study, we investigated the expression of these genes in different tissues of rainbow trout challenged with 4 different inactivated pathogens. By using qPCR, we detected a strong induction of bothcath‐1 and cath‐2 genes within 24 hours after intraperitoneal inoculation withLactococcus garvieae, Yersinia ruckeri, Aeromonas salmonicida, or Flavobacterium psychrophilum cells. Up to 700‐fold induction of cath‐2 was observed in the spleen of animals challenged with Y. ruckeri. Moreover, we found differences in the intensity and timing of gene up‐regulation in the analyzed tissues. The overall results highlight the importance of cathelicidins in the immune response mechanisms of salmonids. |
| sample id | sample name | tissue | strain | treatment | description | |
|---|---|---|---|---|---|---|
| 1. | SRR2598319 | TR_kidney | kidney | Yersinia ruckeri | animal sample from Oncorhynchus mykiss | nan |
| 2. | SRR2598320 | TR_kidney | kidney | Yersinia ruckeri | animal sample from Oncorhynchus mykiss | nan |
| 3. | SRR2598327 | TR_spleen | spleen | Yersinia ruckeri | animal sample from Oncorhynchus mykiss | nan |
| 4. | SRR2598325 | TR_spleen | spleen | Yersinia ruckeri | animal sample from Oncorhynchus mykiss | nan |
| 5. | SRR2598324 | TR_spleen | spleen | Yersinia ruckeri | animal sample from Oncorhynchus mykiss | nan |
| 6. | SRR2598328 | TR_spleen | spleen | Yersinia ruckeri | animal sample from Oncorhynchus mykiss | nan |