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Two groups (wild and domesticated) of fish were collected within the Xin Jiang Province of China. Two biological replicates were collected for wild and domesticated groups. Following anaesthetization (in tricaine methanesulfonate (MS222) at a concentration of 1:18000), each individual was weighed, and then fresh brain, heart, gill, liver, muscle, kidney, and pancreas tissues were quickly collected and immediately stored in liquid nitrogen for RNA isolation. After RNA extraction, RNA from each tissue type were diluted to equal concentrations and then pooled together for each fish individual for RNA-Seq sequencing. |