PROJECT ID: GSE131136


Data source GEO: GSE131136
Description Early and post-embryonic neural crest-derived lineages in the zebrafish trunk in response to thyroid hormone modulation
Key word mitochondrial protein mpv17; neural-crest; beta-galactosidase; pattern-formation; stripe formation; zebrafish; expression; gene; melanocytes; evolution
Publication Saunders LM, Mishra AK, Aman AJ, Lewis VM et al. Thyroid hormone regulates distinct paths to maturation in pigment cell lineages. Elife 2019 May 29;8. PMID: 31140974
Abstract Thyroid hormone (TH) regulates diverse developmental events and can drive disparate cellular outcomes. In zebrafish, TH has opposite effects on neural crest derived pigment cells of the adult stripe pattern, limiting melanophore population expansion, yet increasing yellow/orange xanthophore numbers. To learn how TH elicits seemingly opposite responses in cells having a common embryological origin, we analyzed individual transcriptomes from thousands of neural crest-derived cells, reconstructed developmental trajectories, identified pigment cell-lineage specific responses to TH, and assessed roles for TH receptors. We show that TH promotes maturation of both cell types but in distinct ways. In melanophores, TH drives terminal differentiation, limiting final cell numbers. In xanthophores, TH promotes accumulation of orange carotenoids, making the cells visible. TH receptors act primarily to repress these programs when TH is limiting. Our findings show how a single endocrine factor integrates very different cellular activities during the generation of adult form.


Dataset Information


Dataset ID Species Tissue / Organ Experiment type Sample Source dataset ID
1. GSE131136 (all) Danio rerio trunk, skin baseline 5dpf, sox10:CRE positive cells from 5dpf and post-embryonic zebrafish trunks with and without thyroid hormone GEO: GSE131136

Clustering Result


Cluster Cell type Gene id (symbol) Marker class Evidence
1 Schwann cells ENSDARG00000025670 (fstl3) marker DOI:10.7554/eLife.45181
2 Schwann cell precursor ENSDARG00000009823 (pou3f1) marker DOI:10.7554/eLife.45181
5 Xanthophores ENSDARG00000071475 (aox5) marker DOI:10.7554/eLife.45181
5 Xanthophores ENSDARG00000025912 (bscl2l) marker DOI:10.7554/eLife.45181
6 Melanophores ENSDARG00000056151 (tyrp1b) marker DOI:10.7554/eLife.45181
6 Melanophores ENSDARG00000006008 (dct) marker DOI:10.7554/eLife.45181
7 Pigment progenitors ENSDARG00000008861 (tfap2e) marker DOI:10.7554/eLife.45181
9 Iridophore ENSDARG00000062688 (gpnmb) marker DOI:10.7554/eLife.45181
9 Iridophore ENSDARG00000098745 (tfec) marker DOI:10.7554/eLife.45181
9 Iridophore ENSDARG00000075343 (csf1a) marker DOI:10.7554/eLife.45181
11 Chromaffin cells ENSDARG00000035338 (tppp2) marker DOI:10.7554/eLife.45181
13 Neuron ENSDARG00000102879 (myt1b) marker DOI:10.7554/eLife.45181
14 Proliferating progenitors ENSDARG00000054155 (pcna) marker DOI:10.7554/eLife.45181
15 Neuron ENSDARG00000102879 (myt1b) marker DOI:10.7554/eLife.45181
16 Satellite glia ENSDARG00000025301 (gfap) marker DOI:10.7554/eLife.45181