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BLAST Search Server
Step1: Data Input
Please select the way data input!
Paste sequences in the textbox below
Upload a file
Enter query sequences here in FASTA format
>CI01000000_00050357_00064104 7 exon (s) 50357 - 64104 1584 bp, chain + MTTKQVNAASFNTSAGFNDEWVLHHCECVQLRAEVVALKNIVLKLEKDFNDSVESNLNRE VYFREAVNFKFDKMEESIKDAMATLERKTTNRATDGEFCAPTTTGPDFDIIFSILPFSRQ QLISGQGDDDTLQRLSISPSTPPTNGIDVRNHQGVLYRWIQKGNNNIKIQLIIPRALIQQ TVQHFHQRTAERRHGQLKTLLQVLEVAWWPTIRSDVWRFIGDCKLCGVETKECAVINPSS KPPHNQHHHVHHQHHPPKRPTSRIGGNARGSGVPGRLDATGPWEEWHHRPKDIQAGGYDD LAQTLSTLQLEVDKVHNFLKTMGAAQEENEVLMIFLFSKDLISWSTKACFSCDTRGKVRV LKLDSLVIGCIMRVEEGFDLGENLILYWVSALTWLQYCFASSKSQFVFQFSPTHSLPSQF LSVNLQVWDYNFSKWPEQMSYSISFCVFYEGDRIIRIKLRKFSFYRWMIKYIIHIVWYIS LKVFMRSFIGGGSLLVTKGPALKSAGGSYSSSAPRNDIDISITQGTH >CI01000000_00084533_00088200 3 exon (s) 84533 - 88200 1350 bp, chain + MYVIQEFIHTYVIQTFIHTYVIQMQNLYKNGRVSNIIFYTEDPEAWHSAIRHQAEDTSTHQ STNEQSRDDSEEISLLHSIINDMKEKFREMELVQLREEVHGVAQETDRIERKEDYRKEIAM LKKEIQELKNQREEYNKQVSVIREEMRGLREDRESFishYRKQLPALEKQLTQRGEQQNLEETPS PPTTAINPSSPPPSDSYSQTQPQIKALELLCEEQLGSPSHIIIHTGTNDLRTQQERVATAL INVTEKASSNFPNSKIILSTLLLRSDFHPDTIRHINARVSRDCALKPSVYLAQHPTLNLSS LYDHVHFHRRAVPIFTKTLEDITLNRNSNTQQRSHGHPIEPLQGHPSNHRPLHHFNSERRN PMRGSMFPPGKPHIEGEEAAGLVHSHEHQQEQHSDSQSYAEAVSQSLIHSNTSTVDSQQAS SSSRDLRNIQHMLTLLCSHLMG
Or upload sequence file (Max. 8M)
Sample file download
Step2: Parameter Setting
-p: Program
-- Select a type of BLAST --
blastp
blastn
blastx
tblastn
tblastx
-d: DataBase
-- Select a DataBase --
Grass Carp (genomes)
Grass Carp (cds)
Grass Carp (protein)
Zebrafish (genome)
Zebrafish (protein)
-e: Expected value
-m: Alignment view options
0 = pairwise
1 = query-anchored showing identities
2 = query-anchored no identities
3 = flat query-anchored, show identities
4 = flat query-anchored, no identities
5 = query-anchored no identities and blunt ends
6 = flat query-anchored, no identities and blunt ends
7 = XML Blast output
8 = tabular
9 = tabular with comment lines
10 = ASN, text
11 = ASN, binary
-F: Filter query sequence
T
F
-c: Mask lower case
F
T
-G: Gap open penalty
-1
-E: Gap extension penalty
-1
-M: Matrix
BLOSUM62
PAM30
PAM70
BLOSUM80
BLOSUM45
-P: Identity percentage cut-off
0
30
50
90
Run