AMCG00003589 (col12a1,col12a1a,LOC108428624)


Basic Information

Item Value
gene id AMCG00003589
gene name col12a1,col12a1a,LOC108428624
gene type coding
species bowfin (Amia calva)
category of species ecological fish

Chromosome Information

Item Value
chromosome id CM030121.1
NCBI id CM030121.1
chromosome length 57050121
location 10871341 ~ 10944875 (-)
genome version AmiCal1_2021_bowfin_Genome

Sequence

>AMCG00003589
ATGAAGATCAGGCTGGCGTTGGCAGCAGCTGCCTTGCTGGCAGTGTTGGTTTCCTCAGTCGATGCTCAAGGCATAATTGTCAAGGAGCTAAATCTCCCCTACACTACAACAGAGACCTCAATAACAGATTTAAGTCCGGGTTTAGACTATGAAGTAaccatatattcatattatggATCTGAGGAAAGCATTCCTATCATTGGTCAGCTCACAAAATGTTCAGTGAGTGCCATTGCAGATTTAGTGTTTCTGGTGGATGGATCCTGGAGTGTTGGAAGGGAAAATTTCAAACACATTCGAAGCTTTATTGCTGACCTGGCATCTGCGTTTGACATCGGAGAGGATAAGACAAGAGTTGGAAtcgtgcagtacagtacagacacaagGACCGAGTTCAACctaaatcagtattttaaaagagGCGAACTGCTCCGGGCCATCAACACCTTGCCTTACAAGGGTGGAAACACCATGACAGGTGATGCAATGAACTActtattgaaaaacacatttactgaGCCGGCTGGGGCCAGGAAAGGATTTCCCAAGATTGCAATGGTCATCACAGATGGAAAATCACAGGATCCCGTGGAGGAGTATGCAAGAAAGCTCCGAAACATCGGAGTCGAGATTTTCGTTCTAGGGATCAAGGGAGCAGATGTTGAGGAGCTGAGACAAATGGCCTCTAGTCCCTATAACAAGCATGTCTACAACGTGGCTaactttgatttgattaaaaatgtacagcGTGAGATCATTACCCAAGTGTGTGCAGGTGTCGATGAGCAGCTCAATGAACTTGTGAGTGGAGAAGAAATTATTGAACCAGCCTCCAATTTACAGGTCACAGAAATGTCAGCCAAATCTATGCGGGTAACTTGGGATCCATCCCCCGGCCAAATAACTGGGTACAAGCTGCAGCTCATTCCTATGATATCTGGAAGTGACCGGCAGGAGCTGTACATGGGGCCCTTGGTGACTACCATGACTGTGAAGAACCTTTCTCCCGAAACCGAGTACCAGATTAACTTGTTTGCTCTGAGGGGTTTGACTCCCAGTGAGCCTGTGATGGCCATGGAGAAAACTCAGCCTGTGAAAGTGTCACTGGagTGTTCTCTTGGAGTGGATGTTCAAGCAGATGTTGTTCTCTTGGTCGATGGCTCGTACAGCATAGGATTAGCAAATTTTGCCAAAGTGAGAGCTTTCCTGGAAGTCTTAGTGAACTCCTTTGACATCGGACCCAACAAAGTACAGATCAGTCTTGTCCAATACAGCAGAGATCCCTACACCGAATTTGCTCTGAACACCCACCATGACATGTCCGCTGTGGTCAAAGCAGTTCGGACCTTCCCGTACAGAGGAGGCTCGACAAACACAGGCAAAGCCATGACCTACGTGAGAGAGAAGATATTCATTCCCAGCAAAGGAGCCCGGCCCAACGTGCCTAGAGTCATGATCCTCATCACTGACGGCAAATCGTCGGACGCCTTCAAAGACCCCGCCACTAGGCTACGCAGCACTGACGTAGAGATATTTGCAGTGGGTGTCAAAGATGCCGTTCGATCAGAGCTGGAGGCCATCGCTAATCCCCCTGCAGACACTCATGTGTACACCGTGGAGGACTTTGACGCTTTTGAAAGGATATCCAAGGAGCTCACCCAGTCTATCTGCTTGAGAATTGAGCAGGAACTCAAGATGATTCTGATGAGAActttaagcCCTCCAAGGGACCTCCAGTTCTCTGACGTGACATCCAGAAGTTTCCATGCCAGCTGGTCTTCCGACGCACCGAATGTTATGTCCTTCCTTGTCAGGTTCAGGCCAGTGGCTGGTGGAGATTACGTCTCCATTGCCGTCCCCGCAGACACCACGTCCACGATCTTGCCTCATCTCACCCCTTTGACCAAGTATGAAGTTGAGGTTTACTCCCAATATGAGAGGGGAGACAGCTTTCCCTTGAAAGGAGAGGAGACCACTCTAGAAGAACAAGGAACTCCTCGTAATCTCCGGGTGTCTGAGGAGACGACGGACAGTTTCAAAGTGACGTGGGAAGCAGCACCTGGTCCAGTTGTAAGGTATCGCCTCTCCTACATTCCCACcagtggagagggagagaggaaggagacGGCCACCAGCGGAACCGAGACCTCTATCGTGCTCCATGACCTCCTTCCCCTGACCACATATCATGTATCCGTGTCTGCAGAATATCCCTCAGGGGTTGGAAATGAGATGCAGATTGACGGAATGACTAAAGAAGccAGGGGATCCCCAAGAGACCTGCAGGTTTATGACAACACCGTCTCGAGCATGAGGCTGTCCTGGGAGGCTGCCCCAGGGCAAGTCTTGCAGTACCGGGTGGCATACAGGCCAGCCGctgggggagagaaaaaagagaTCTCTGTCAAGGGTGATGTCACCACCGCGTTGCTGAAGAACCTGCAGCCAGACACGGAGTATGAGCTGTCTGTGAGCGCCCGCTACGCCTCAGGACTCGGGGACCCCCTCAATGGACAAGGAACTACACTGGAAGAACTTGGTTCCCCCCGGGATTTGGTGACAAAGGATGTCACTGACACAAGCTTTGGAGTCTCATGGGCAGCGGCACCGGGGAAGGTTCGGGCTTACAGGATAGCCTGGAAATCACTTTTCACCGAGGAGGCCGGAGAGAAAAGCATTCCCGGAGACGAGACCGAGACCGTGCTGGAGAACTTGACGCCTGAAACCAAATACCAGGTGTCTGTCTATGCAGCATACGGCCGCGGGGAAGGGAATCCATTGGTGGGAGAGGAAACAACAGATGCTTCCGCAGCTGCCAAGACCTTAACCGTGTCTGAGGAAACTGAGAAAAGTATGAAGGTGACATGGGAGCCGGCGCCTGGCAATGTGGTGAACTACAGGGTCAGCTACCGACCAACGGCTGGCGGGCGACAGCTGGCCACCAAGGTGCCTGGGACCATGACAAACACTGTGCTCCGGCGCCTAAAGCCCCTGACCTCATACGACATCATCGTGAATCCGGTCTACAGGCGTGGAGAAGGAAAAGCAAGGCAAGGAGTAGGAGTGACATtgtCACCTTACAAAGGCCCCAGGAACCTACAAACCTCTGAGCCAACTAAGACCAGTTTCCGAGTGAGCTGGGACCACGCCCCGGGAGATGTGCGAGGCTATAAGGTCACCTTCCACCCAACTGGAGAAGACATTGATCTGGGGGAGCTGCTTGTGGGACCCTATGACAACACAGTTGTCCTGGAAGAGCTGAGGGCTGGAACTAAATacacagtcagtgtgtttgGCATGTTTGATGGTGGAGAAAGTATGCCACTCGCTGGTGAAGAAAAGACAACCCTTATGGATGCACCTGAACCCCCTCCGCTTGATAACCTAGATGTCAAGTGTAAGACCACGGCTCAGGCCGACATCGTCCTGCTGGTTGATGGATCCTGGAGTATCGGACGACTGAACTTCAAAACCATCAGGTCCTTCATTGCCCGTATGGTCGGAGTCTTTGATATCGGTCCTGACAGAGTCCAGATTGGTCTGGCTTTGAATTACATTCTTCAGAACAACTTCAAGCCCAACGTAGGACTCCGGCCCAATTCACGCAAGATAGGCGTGCTCATCACTGATGGCAAATCACAAGATGACATCATCGAAAACTCCCAAAACCTGCGAGATCAGGGCATTGAGCTGTACGCTATTGCTGCTGCAACAGCAGAAGCCCCAACAGACCTGGTGACATCTGAAGTCACAACTCGATCATTCAGGGCAAGCTGGACTGCGCCCGGCAGTTCCGTGGACAAGTACCGTGTTCAGTACATGCCTGTGGCTGGAGGCAGGTCTAAAGAGATATTTGTTGATGGATCAGTTACAACAACAATCCTTCCTGATCTGACTCCTCTGACGGAGTATTACGTCAATGTTTATGCCATCCTTGGGGAGCAAAGCAGCGAGCCCTTAAAAGGCACAGAAACCACTCTGCCCCTCGCAGCGGTGAGGAAAATGAATACCTATGACGTGAGCCCAACTACTATGAGAGTGAGGTGGGAGGAAGCCAGTGAGGCCAGTGGCTACATGTTGCTGTACAGCCCAATCAATGCTACAGTACCAGCAGGGGAGAAGGAGCTGAAAGTTGGGTATGATGAGACTGATGTTCAGCTGGTCGAGCTGATTCCCAACACTGCGTACACACTCTCCTTGTTTGCCCTCTATGGAGACGCAACTAGTGATCCTCTAACAACACAGGATGTGACGTTGCCATTACCACCAGCAGGGGAGCTGAGGATCTCCGATGTCACACACAGCACTATGAACCTTAACTGGGATGCGGCACCAGGAAATGTTCGCAAATATATCATCACGTACAAGCCTGTCGATGGAGATGAAGCCAAGGAGATAGAGGTCGGAGGCAGGACAACTACAAAGGTCCTCGATAACCTCATATCTCAGACTGAGTATGACGTGGCAGTCACTCCTGTATACGATGAAGGGCCTGGAAGTCCCATGCTTGGACAAGCTGTGACAGATGTCGTCCCCGCACCGAGAAACCTGAGATTCTCTGAAGTGACATCGACCAGTTTCAGGGCCACGTGGGATCATGCAGCTCCTGACGTCGCTCTCTATAGAGTTGGCTGGGTTAAACAGGGCGACACCGACAGACAATTTGCGATCCTCAACAGCGATGACAACTCCCAAGTCCTAGAGAACCTGGATCCAGACACAATGTATGAAGTTCAGGTCACAGCCATCTACCCAGATGAATCGGAGAGCGAGGATTTAATGGGCAGACAGCGCACACTGCCAATAAAGGTTATAACACCTGCTCCCACAGGTCCCCCTCAGAATATGCAGGTGTACAACGCTACCACGAGCAGCTTAACGGTGAAATGGGACCACGCGCCAGGGAAGGTGCAGAACTACAGGATAACCTATGTCCCTACAGCTGGGGGGCAGCCACAGTCAGAGCAAGTGTCTGGTGGCACAACATCTACTGTTCTGAAGAACCTTCTCTCAGACACGCTCTACAAGGTGACAGTGGTCCCTGTGTATCCTGAGGGGGAGGGCAAACGTctctctgaaaatgaaaagacatTGGTTCGCAGCCCACCCCGCAATATTGAAGTCTTCAATCCAACTCCGAACAGCCTCAATGTGCGCTGGGAGCCTGCGTCGGGCCAAGTCCAGCAGTATCGCGTCACCTATGCTCCCCTGACGGGAACAAGGCCTTCTGAATCTGTGTTGGTGCCAGGAAACACGCACACTGCATTCCTCCAACAGCTCATCCCAGACACTCCTTACAATGTCAATGTTGTGGCTCTCTACTCCGACGGAGAGGGAGCCCCAGTTCCAGGAGATGGGAAAACACtgCCACGCAGTGGACCAAGAAACCTCCGTGTATTTGGTGAAACCACAAGCACTCTGAGTGTCAGCTGGGATCATGCCGAGGGCCCCGTGCAGCAGTACAAAATCACCTACGCTCCCACGACTGGAGACCCGATCGAAGAGTTCACTGTTGTTCCTGGAAGAAGAAACACCGTCACCCTGCAGAACCTGCAGGCTGACACCCCTTACAGGATCAATGTGGTGGCTGTGTACCCTGATGGCGCAGGTGGTGAGCTCACCGGAAATGGAAAGACAGTCGGTCTGCTGGAGCCCCGCAATCTGCGCGTGTCGGACGAGTGGTACACCCGCTTCCGGGTCTCCTGGGATCCTTCCCCGTCGCCAGTCCTGGGATACAAGCTTGTCTACCAGCCCACAGATAAGGATGAAACGGTGGAAGTGTACGTCGGCGATGTCACAACGTATGTCTTGCACAATCTGAACCCTGGCACTGTTTATGATGTCAAAGTCTACTCTCAGTATGACGGGGGAGTGAGTGGTGCTCTGACAGGACAAGGAACCACATTGTACCTCAACGTCACTGACCTTAAAACACACACCGTTGGCTTTGACAGGTTCTGCATCCAGTGGCTTCCTCATAGATCAGCCACTTCTTACCGAATTAAGCTGAATCCTCTTGATGTGTACAGCCTTGGACAGCAGGAGGTCACTGTTAACGGGGGAGAGACAAATTATTGCTTCAGTGGCCTCTCTCCTGATGCCTTATACAATGCCACTGTCTTTGCCCAGACACCCAACCTTGAAGGAGCTGGGGTTAGTGTGAGAGAGCGGACCTTGGTTAAACCCACAGAAGCTCCCACTGAGCCCCCAaccccacctccacctcccacTATTCCACCTTCCTGGGAAGTTTGCAAAGGTGCCAAGGCAGATCTGGTGTTCCTGATTGATGGATCTTGGAGTATCGGTGATGATAACTTCCACAAAgttctgcagtttgtttttgacACCACTGGAGCCTTTGACGTCATCAGTCCAAATGGAATGCAGGTGTCTTTCGTGCAGTACAGTGATGACGCAAAGACCGAATTTAAACTCAACACTTACAAAGACAAGGGCATGGTTCTATCAGCTCTCAAACTTGTCCGTTACAGAGGAGGAAACACCAGAACAGGTGTAGCTCTGAAGTACATCGGTGAGAAGGTCCTGACCCCAGAGAATGGAATGAGGAAGAACGTTCCAAAGGTGCTCGTTGTTGTCACCGATGGCCGGTCACAAGATGAAGTGAAGAAAACTGCCCCTGCATTACAGCATGCTGGCTACAGTGTGTTCGTTATTGGAGTTGCTGATGTGGATTACTCAGAGCTGCAGCAGATTGGAAGCAAGCCCAGTGACCGTCATGTCTTTGTCGTGGATGACTTTGACGCTTTTGAAAAGATTCAGGATAATCTCATTACATTCATCTGTGAGACCGCAACCTCAACTTGTCCTTTGATTTACCTTAATGGGTACACCTCACCAGGCTTCAGAATGCTTGAGGCGTTCAACCTCACAGACAAGACGTTTGCCTCCGTAAAAGGGGTGTCTATGGAGCCAGGATCTTTCAACAGTTTTACGGCCTACAGGCTCCATAAAAACGCATACCTCTCGCAGCCCACAAAAGAGGTCCACCCAGAAGGCCTGCCACCTGCCTACACCCTGATCCTGCTGTTCCGCCTTCTCCCTGACACCCAGACCGAGGCGTTTGACATGTGGCAGATTTCAGATAGCAGCAACAAACCCGAAGTGGGGCTCACAGTTGATCctTCCAGCAAAACATTATCTTTTTACAATAAGGACACGAGGGGAGAGATCCAGAAGGTTACGTTTGATAACgatgaagtgaagaaaatctTCCACGGAAGCTTCCACAAGctTCACATAGCTGTCTCCCCCAAGagtgtcaaaataaatgtggattGCCAGGAAGTGGCAGAGAAAGAAATCAAGGAAGCCACCAACATTTCTACTGATGGCTATGAAGTTCTTGGCAAAATGGTGAAGTCGAGGGGATCCCAAAGAGAGTCGGCCACATTCCAGCTCCAGATGTTTGATATTGTTTGCAGCTTAGGCTGGACCAGCAGAGACAGATGTTGCGACCTGCCATCAATGTCTCTAATGAAagagaAGAGGCGAAATGTCCCGCTCTGCCTCATGCCTGCACCTGTGCTCAGGACAGCATTGGGCCACCGGGCCCTGCTGGACCTCCGGGGGGACCTGGTAGCAAAGGACCTCGAGGTGAAAGAGGAGAAACTGGCCAAGCTGGCCCAGTCGGCACACGTGGGGAGCCAGGGTTACCAGGCCCTATGGGACTTCCAGGCCCTCAAGGACCCAATGGACTGTCTATTCCAGGAGAACCGGGTCGGCAAGGACCGAAAGGTGATGCTGGTGATCCGGGCCTACCTGGACGTTCAAAGTAACCTGTTCTTTGGAGTGTTGAAGGACTGTTTAGAAATGATGGACATCAgGACCTCCTGGCCCACCTGGACCATTAGGACCTGTTGGCCCAGTAGGAGGAAGGGGACCTCAAGGAAAGGAAGGGCCTGCGGGACCCAGGGGCCCACCTGGACCAATGGGGGCTCCGGGATCACCTGGCATGCCTGGATCAAGTGGGAAAACAGGGAAACCAGGAGACTCAGGGCAGCCTGGGCCAGCTGGCATgaaaggagagaaaggggagaggGTGAGTAtaattgtggtgttttttttgtgtgcgcTGGTGTTTTGAGGAGCACGTATGACTTGATCCTAATCAAAAACTGCTTCTTGCAGGGAGACTTTGCATCCCAGAATATGATGCGCTCGATTGCCAGACAAGTCTGTGAGCAGCTGATGAATGgcCAAATGAGCAGGATTGACACTATGCTGAACCAAATTCCCAATGGCTATGTTACTCGCAACAACCCCGGCCCTCCTGGCCCCCCTGGTCCCCCTGGCAGTTCTGGTCCTCAAGGTGAACAAGGACAAGCAGGCCAGAACGGATTCCCAGGAAGTCCAGGCACACCTGGCCGCCCAGGAGACAGAGGTCCACCAGGAGAGAAGGGTGAACGGGGCAGTCCAGGAAAAGGAACCCAGGGACAACGAGGTTTAACTGGACCGCCTGGCCCACCAGGAGAATCTCGAACTGGCCCACCAGGTCCTCAAGGCTCAACAGGGCCTCGTGGTCCACCTGGTCGCCAGGGCCAGGCAGGAGTCAGGGGGCCACCCGGTCCCCCAGGTTACTGTGACTCTTCACAGTGTGTTGGCATTCCTTACAATGGTCAAGGATACCCAGAACCATACCCGCCTGAGCCCGAAACCTATGTAGTTTCCATTCCAGAGGAGCAGCCCGACGACtatgaaacagaaatacagtCCCCCGGTTATTCGCGAAACCAGCGCGGAAAGAGGTCACTATCAAGACAGCAATACGCAAGGATAgcaccataaaaaaaaacaggattgtGTTCTTTTTATGGGAAGTCATTTGCTATTTTCACACATGCTGGTGTTGACAGAATTGGAAGGAAATGTATGAGGGTTTGATACATTTCATAGACGCATGTGTACATTTAGCGAACAAAAGAAATGGCCAAAACATACAAcgcaaaacaaaacccaaacatTATTTCTTAAATGAAACAACCTGTGAGCTTGCTATATGTTCCCTTAAAGTGTCTTTGCTTGTAATATAACTGCCTTAAGTGTATACTAAAGAGTCTAATGTTTAATGCTCTCATCATGTCaaattcagcattttaaaacatccgGAAGAAAGGTTACATTTGTTTGCAGCATGAGGGTATCTAGCAATAAATGGCATGGATCCTTCCCCTTTTGTACATCACCACAAATGTCTTATTGTAAagctgtttgttgttgtaatggaattatatattttgcctCTTTATCTGTCATTGTAAAGAGGAGACTTTGTACAAGATCTACCTACAGTTCACAAAACGTCAATACAGCAGTTAAACATgactttcattttgttattttagactaaatatttaagttttattatACCAACTCACAGCTGATTTTACCAAAGGTGGAATTGCATGTCTGTGTTGTATATTTAGTATGACTGTTAAATTTTTTTATCAGAAtataggatgtttttttttggtttgtttttttcagcgATATTCAATGCAGTTTGAATTTGTGTGCAAGACTATGGACATAGAATTGcttctttcatgtttttaactgcaaattgtgaatttcacagcc

Function

symbol description
col12a1a Acts upstream of or within regeneration and regulation of axon regeneration. Predicted to be located in extracellular region. Predicted to be part of collagen trimer. Predicted to be active in collagen-containing extracellular matrix and extracellular space. Is expressed in several structures, including immature eye; integument; myoseptum; nervous system; and trunk vasculature. Human ortholog(s) of this gene implicated in Ullrich congenital muscular dystrophy. Orthologous to human COL12A1 (collagen type XII alpha 1 chain).
col12a1 Predicted to be an extracellular matrix structural constituent conferring tensile strength. Involved in endodermal cell differentiation. Located in extracellular exosome. Colocalizes with collagen-containing extracellular matrix. Implicated in Ullrich congenital muscular dystrophy.

NR:

description
PREDICTED: collagen alpha-1(XII) chain

GO: NA

KEGG:

id description
K08132 COL12A; collagen type XII alpha

RNA

RNA id representative length rna type GC content exon number start site end site
AMCG00003589 True 9600 mRNA 0.50 60 10871341 10944875

Neighbor

gene id symbol gene type direction distance location
AMCG00003584 NA coding downstream 254344 10584660 ~ 10616997 (-)
AMCG00003582 NA coding downstream 291016 10578898 ~ 10580325 (-)
AMCG00003579 LOC107197779 coding downstream 480306 10376843 ~ 10391035 (-)
AMCG00003577 NA coding downstream 554502 10278240 ~ 10316839 (-)
AMCG00003578 xrn2,LOC106571262,LOC108229358,LOC104951730 coding downstream 598574 10236749 ~ 10272767 (-)
AMCG00003588 tmem30ab,LOC107721815,LOC107658180,LOC107597847,LOC107714749,LOC108257743,LOC107664818 coding upstream 12534 10957409 ~ 10963201 (-)
AMCG00003592 NA coding upstream 25489 10970364 ~ 10996825 (-)
AMCG00003596 impg1 coding upstream 205194 11150069 ~ 11183677 (-)
AMCG00003597 htr1b,LOC103360562 coding upstream 304212 11249087 ~ 11250247 (-)
AMCG00003600 NA coding upstream 401069 11345944 ~ 11366600 (-)
G1744 NA non-coding downstream 455454 10414938 ~ 10415887 (-)
G1631 NA non-coding downstream 1019003 9852069 ~ 9852338 (-)
G1630 prkd3,LOC107098152,LOC106607674 non-coding downstream 1019914 9850691 ~ 9851427 (-)
G1492 NA non-coding downstream 1691360 9174855 ~ 9179981 (-)
G1415 cpsf3 non-coding downstream 1893492 8974810 ~ 8977849 (-)
G1856 NA non-coding upstream 105276 11050151 ~ 11051078 (-)
G1935 NA non-coding upstream 436997 11381872 ~ 11386054 (-)
G2044 NA non-coding upstream 787929 11732804 ~ 11863101 (-)
G2052 LOC100846954 non-coding upstream 914046 11858921 ~ 11905671 (-)
G2066 NA non-coding upstream 1082044 12026919 ~ 12027158 (-)
AMCG00003587 naa20 other downstream 238229 10625346 ~ 10633112 (-)
AMCG00003573 NA other downstream 992897 9874670 ~ 9878444 (-)
AMCG00003572 NA other downstream 1005692 9853199 ~ 9865649 (-)
AMCG00003550 NA other downstream 1788491 9009117 ~ 9082850 (-)
AMCG00003484 NA other downstream 5514873 5344070 ~ 5356468 (-)
AMCG00003586 NA other upstream 6209 10951084 ~ 10956353 (-)
AMCG00003595 myo6,LOC106571274 other upstream 118246 11063121 ~ 11116905 (-)
AMCG00003618 NA other upstream 799114 11743989 ~ 11789275 (-)
AMCG00003623 LOC101472980,LOC105894864,LOC108427479,LOC105908251,LOC106571292,LOC105017864 other upstream 977116 11921991 ~ 11939783 (-)
AMCG00003625 NA other upstream 1130444 12075319 ~ 12078152 (-)

Expression


Co-expression Network

Homologous

species gene id symbol gene type chromosome NCBI id location
grasscarp (Ctenopharyngodon idella) CI01000029_06606850_06665941 NA coding CI01000029 null 6606850 ~ 6666150 (-)