PROJECT ID: GSE148591

Data source GEO: GSE148591
Description We applied two strategies for labeling the endoderm derived cell in the pituitaries of zebrafish. The contribution of endoderm derived cells and the cell heterogeneity was later analyzed and resolved through constructing the single cell transcriptome through 10X Genomic pipeline. The first strategy is through endodermal lineage tracing techniques by Sox17 driven Cre recombinase conjuncted with an estrogen receptor (Sox17:CreERT2), which allows us to induce the conversion by addition of tamoxifen. We are able to identify the endoderm derived cell by microscopic observation with expression of mCherry gene from EGFP background. We made two single cell transcriptomic libraries by this strategy: the males library (file names started with M_Pituitary_Sox17creERUbiGtR) was derived by pooling 6 adult zebrafish pituitaries with moderate Sox17:CreERT2 conversion rate. The male1 library (file names started with M1_Pituitary_Sox17creERUbiGtR) was derived from the pituitary from a male zebrafish with high conversion rate. The second strategy is through transplant of taram-a mRNA injected GFP+ embryo to limit the cell into endoderm lineage. The treated embryonic cells were then injected into a BFP+ wild type embryo for tracing. The endoderm derived cells can be identify by expression of GFP. We made one single cell transcriptomic library by this strategy: the ubeGFP_transplanted (file names started with ubieGFP_Pit-Endo-transp) library was derived from pooling of pituitaries from adult transplanted fish.
Key word amphioxus; origin; component
Publication Fabian P, Tseng KC, Smeeton J, Lancman JJ et al. Lineage analysis reveals an endodermal contribution to the vertebrate pituitary. Science 2020 Oct 23;370(6515):463-467. PMID: 33093109
Abstract Vertebrate sensory organs arise from epithelial thickenings called placodes. Along with neural crest cells, cranial placodes are considered ectodermal novelties that drove evolution of the vertebrate head. The anterior-most placode generates the endocrine lobe [adenohypophysis (ADH)] of the pituitary, a master gland controlling growth, metabolism, and reproduction. In addition to known ectodermal contributions, we use lineage tracing and time-lapse imaging in zebrafish to identify an endodermal contribution to the ADH. Single-cell RNA sequencing of the adult pituitary reveals similar competency of endodermal and ectodermal epithelia to generate all endocrine cell types. Further, endoderm can generate a rudimentary ADH-like structure in the near absence of ectodermal contributions. The fish condition supports the vertebrate pituitary arising through interactions of an ancestral endodermderived proto-pituitary with newly evolved placodal ectoderm.


Dataset Information

Dataset ID Species Tissue / Organ Experiment type Sample Source dataset ID
1. GSE148591 (6mpf) Danio rerio pituitary baseline 6mpf, male, Sox17:CreERT2 GEO: GSM4474603
2. GSE148591 (3mpf) Danio rerio pituitary transplant 3mpf, graft transplantation during early embryogenesis GEO: GSM4474605

Clustering Result

Cluster Cell type Gene id (symbol) Marker class Evidence
1 Somatolactotropes ENSDARG00000017251 (smtlb) marker DOI:10.1126/science.aba4767
1 Somatolactotropes ENSDARG00000038191 (smtla) marker DOI:10.1126/science.aba4767
2 Lactotropes ENSDARG00000037946 (prl) marker DOI:10.1126/science.aba4767
3 Corticotropes ENSDARG00000043135 (pomca) marker DOI:10.1126/science.aba4767
3 Corticotropes ENSDARG00000079187 (tbx19) marker DOI:10.1126/science.aba4767
4 Somatotropes ENSDARG00000038185 (gh1) marker DOI:10.1126/science.aba4767
5 Gonadotrope ENSDARG00000099363 (lhb) marker DOI:10.1126/science.aba4767
6 Melanophores ENSDARG00000043135 (pomca) marker DOI:10.1126/science.aba4767
6 Melanophores ENSDARG00000100398 (pax7a) marker DOI:10.1126/science.aba4767
7 Corticotropes ENSDARG00000043135 (pomca) marker DOI:10.1126/science.aba4767
8 Thyrotropes ENSDARG00000033726 (tshba) marker DOI:10.1126/science.aba4767
9 Prop1+ group ENSDARG00000039756 (prop1) marker DOI:10.1126/science.aba4767
10 Gonadotrope ENSDARG00000010841 (fshb) marker DOI:10.1126/science.aba4767
11 Isl1+ group ENSDARG00000004023 (isl1) marker DOI:10.1126/science.aba4767
12 Pituicytes ENSDARG00000077121 (cyp26b1) marker DOI:10.1126/science.aba4767

Cluster Cell type Gene id (symbol) Marker class Evidence
1 Somatolactotropes ENSDARG00000017251 (smtlb) marker DOI:10.1126/science.aba4767
1 Somatolactotropes ENSDARG00000038191 (smtla) marker DOI:10.1126/science.aba4767
2 Somatotropes ENSDARG00000038185 (gh1) marker DOI:10.1126/science.aba4767
3 Gonadotrope ENSDARG00000099363 (lhb) marker DOI:10.1126/science.aba4767
4 Lactotropes ENSDARG00000037946 (prl) marker DOI:10.1126/science.aba4767
5 Corticotropes ENSDARG00000079187 (tbx19) marker DOI:10.1126/science.aba4767
5 Corticotropes ENSDARG00000043135 (pomca) marker DOI:10.1126/science.aba4767
6 Gonadotrope ENSDARG00000099363 (lhb) marker DOI:10.1126/science.aba4767
7 Melanophores ENSDARG00000043135 (pomca) marker DOI:10.1126/science.aba4767
7 Melanophores ENSDARG00000100398 (pax7a) marker DOI:10.1126/science.aba4767
8 Gonadotrope ENSDARG00000010841 (fshb) marker DOI:10.1126/science.aba4767
9 Thyrotropes ENSDARG00000033726 (tshba) marker DOI:10.1126/science.aba4767
10 Somatotropes ENSDARG00000038185 (gh1) marker DOI:10.1126/science.aba4767
11 Prop1+ group ENSDARG00000039756 (prop1) marker DOI:10.1126/science.aba4767
12 Isl1+ group ENSDARG00000004023 (isl1) marker DOI:10.1126/science.aba4767
13 Endothelium ENSDARG00000054632 (fli1a) marker DOI:10.1126/science.aba4767
13 Endothelium ENSDARG00000105215 (kdrl) marker DOI:10.1126/science.aba4767
13 Endothelium ENSDARG00000076972 (plvapa) marker DOI:10.1126/science.aba4767
14 Pituicytes ENSDARG00000077121 (cyp26b1) marker DOI:10.1126/science.aba4767