FishCODE

Background Data Information

This section provides a reproduction of the data information you selected in the FishCODE methylation analysis section.

Condition	Project Name	SRA Name	Treatment	Age	Tissue	Sex
treatment_0	User's dataset	meth_example_01_SRR12967081	control	null	null	null
treatment_01	User's dataset	meth_example_02_SRR12967082	hypoxia	null	null	null
treatment_01	PRJNA673886	SRR12967084	hypoxia_meth	null:null	sperm	null
treatment_0	PRJNA673886	SRR12967081	control_meth	null:null	sperm	null

This section provides a reproduction of the analysis parameters you selected in the FishCODE Methylation Analysis section.

1. Remove batch effect Selecting ways to treat potential batch effects: remove batch effect related principal components

Remove batch effect The explanation degree of this principal component is lower than 0.2 and is significantly less correlated with batch processing than 0.01

2. Filter low-quality location site more than 3 methylated reads and coverage lower than 99.9

3. Tile window analysis parameters The window size is 1000, tStep size is 1000 and minimum number of bases to be covered in a given window 10

4. Differential Analysis Parameters qvalue is 0.01, difference is 25 and type is all

5. Distance of associated genes 2000

6. The email for receiving results:

Running results and downloads

Operating Status this status is automatically refreshed in the background every ten seconds (10s) (so the page should respond about 10s later than the email)

Your current task progress: The Platform is checking the operational status of the task, please wait

The normal development vocabulary is: 1."Calculating differences" -> 2."Annotating related genes" -> 3."Annotating upstream and downstream associated genes" -> 4."The calculation has been completed and the visualization file is being configured" -> 5."All steps have been completed, waiting for the results to be displayed"

If an error occurs, the status vocabulary is: "Your sequencing was terminated for unknown reasons, please contact the author in time."

DMRs Information

-Waiting for the results, this process usually lasts 3 hour to 5 hour

Result download