PROJECT ID: PRJNA608912

Data source SRA: PRJNA608912
Description Cardiac regeneration occurs primarily through proliferation of existing cardiomyocytes, yet the regenerative response also involves complex interactions between distinct cardiac cell types including not only cardiomyocytes, but also non-cardiomyocytes (nonCMs). However, the molecular features and cellular functions of the highly heterogeneous populations of nonCMs and how these populations cooperate to regenerate the injured heart remain largely unexplored. Using the newly developed LIGER algorithm that allows flexible modeling across highly diverse single-cell datasets, we analyzed the transcriptome dynamics of 61,977 individual nonCMs isolated at multiple time points during zebrafish heart regeneration. Combining single-cell analysis and in situ hybridization, we identified major nonCM cell types, including multiple novel subpopulations with unique tempo-spatial distributions and highly cooperative interactions in the regenerating heart. Interestingly, genetic perturbation of macrophage function by kit knockout led to accumulation of fibrotic deposits and severely compromised cardiomyocyte proliferation and myocardium regeneration. Our single-cell transcriptomic analysis of nonCMs during cardiac regeneration provides a blueprint for interrogating the molecular and cellular basis of cardiac regeneration.
Key word heart regeneration; non-myocytes; scrna-seq; topologizer; zebrafish; cell transcriptome analysis; epicardial cells; expression; gene; differentiation; inflammation; macrophages; reveals; dedifferentiation; cardiomyocytes
Publication Ma H, Liu Z, Yang Y, Feng D et al. Functional coordination of non-myocytes plays a key role in adult zebrafish heart regeneration. EMBO Rep 2021 Nov 4;22(11):e52901. PMID: 34523214
Abstract Cardiac regeneration occurs primarily through proliferation of existing cardiomyocytes, but also involves complex interactions between distinct cardiac cell types including non-cardiomyocytes (non-CMs). However, the subpopulations, distinguishing molecular features, cellular functions, and intercellular interactions of non-CMs in heart regeneration remain largely unexplored. Using the LIGER algorithm, we assemble an atlas of cell states from 61,977 individual non-CM scRNA-seq profiles isolated at multiple time points during regeneration. This analysis reveals extensive non-CM cell diversity, including multiple macrophage (MC), fibroblast (FB), and endothelial cell (EC) subpopulations with unique spatiotemporal distributions, and suggests an important role for MC in inducing the activated FB and EC subpopulations. Indeed, pharmacological perturbation of MC function compromises the induction of the unique FB and EC subpopulations. Furthermore, we developed computational algorithm Topologizer to map the topological relationships and dynamic transitions between functional states. We uncover dynamic transitions between MC functional states and identify factors involved in mRNA processing and transcriptional regulation associated with the transition. Together, our single-cell transcriptomic analysis of non-CMs during cardiac regeneration provides a blueprint for interrogating the molecular and cellular basis of this process.


Dataset Information

Dataset ID Species Tissue / Organ Experiment type Sample Source dataset ID
1. PRJNA608912 (wt_unjuried) Danio rerio heart baseline 5mpf, wild type, uninjured SRA: SRR11186432
2. PRJNA608912 (wt_2dpi) Danio rerio heart regeneration 5mpf, wild type, 2 days post injury SRA: SRR11186440
3. PRJNA608912 (wt_7dpi) Danio rerio heart regeneration 5mpf, wild type, 7 days post injury SRA: SRR11186448
4. PRJNA608912 (wt_14dpi) Danio rerio heart regeneration 5mpf, wild type, 14 days post injury SRA: SRR11186452
5. PRJNA608912 (kit_uninjuried) Danio rerio heart regeneration 5mpf, kit mutant, uninjured SRA: SRR11186457
6. PRJNA608912 (kit_2dpi) Danio rerio heart regeneration 5mpf, kit mutant, 2 days post injury SRA: SRR11186464
7. PRJNA608912 (kit_7dpi) Danio rerio heart regeneration 5mpf, kit mutant, 7 days post injury SRA: SRR11186472
8. PRJNA608912 (kit_14dpi) Danio rerio heart regeneration 5mpf, kit mutant, 14 days post injury SRA: SRR11186480

Clustering Result

Cluster Cell type Gene id (symbol) Marker class Evidence
1 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
2 Fibroblasts ENSDARG00000006526 (fn1b) marker DOI:10.1016/j.ydbio.2013.08.012, DOI:10.15252/embr.202152901
2 Fibroblasts ENSDARG00000012405 (col1a1a) marker DOI:10.1172/JCI74783, DOI:10.15252/embr.202152901
3 Endocardial endothelial cells ENSDARG00000045141 (aqp8a.1) marker DOI:10.15252/embr.202152901
5 Mesenchymal cells ENSDARG00000027582 (angptl7) marker DOI:10.1186/s12862-016-0859-x, DOI:10.15252/embr.202152901
5 Mesenchymal cells ENSDARG00000104340 (rspo1) marker DOI:10.1242/dev.068460, DOI:10.15252/embr.202152901
5 Mesenchymal cells ENSDARG00000086189 (mgp) marker DOI:10.15252/embr.202152901
6 Macrophages ENSDARG00000090783 (mfap4) marker DOI:10.1371/journal.pone.0138949, DOI:10.15252/embr.202152901
6 Macrophages ENSDARG00000044612 (c1qb) marker DOI:10.15252/embr.202152901
6 Macrophages ENSDARG00000009087 (cd74a) marker DOI:10.15252/embr.202152901
7 Coronary endothelial cells ENSDARG00000002644 (rgs5a) marker DOI:10.15252/embr.202152901
7 Coronary endothelial cells ENSDARG00000014646 (aoc2) marker DOI:10.15252/embr.202152901
7 Coronary endothelial cells ENSDARG00000070038 (rbp2a) marker DOI:10.15252/embr.202152901
9 Immune cells ENSDARG00000105263 (ccl36.1) marker DOI:10.15252/embr.202152901
9 Immune cells ENSDARG00000020788 (sla2) marker DOI:10.15252/embr.202152901
10 Erythroid cells ENSDARG00000011166 (cahz) marker DOI:10.1182/bloodadvances.2018020156, DOI:10.15252/embr.202152901
13 Neutrophils ENSDARG00000057789 (lyz) marker DOI:10.1189/jlb.4MR1114-524R, DOI:10.15252/embr.202152901
13 Neutrophils ENSDARG00000019521 (mpx) marker DOI:10.1189/jlb.4MR1114-524R, DOI:10.15252/embr.202152901

Cluster Cell type Gene id (symbol) Marker class Evidence
1 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
1 Endocardial endothelial cells ENSDARG00000045141 (aqp8a.1) marker DOI:10.15252/embr.202152901
2 Macrophages ENSDARG00000044612 (c1qb) marker DOI:10.15252/embr.202152901
2 Macrophages ENSDARG00000009087 (cd74a) marker DOI:10.15252/embr.202152901
3 Fibroblasts ENSDARG00000006526 (fn1b) marker DOI:10.1016/j.ydbio.2013.08.012, DOI:10.15252/embr.202152901
3 Fibroblasts ENSDARG00000012405 (col1a1a) marker DOI:10.1172/JCI74783, DOI:10.15252/embr.202152901
5 Coronary endothelial cells ENSDARG00000070038 (rbp2a) marker DOI:10.15252/embr.202152901
6 Macrophages ENSDARG00000089362 (grn1) marker DOI:10.15252/embr.202152901
7 Fibroblasts ENSDARG00000006526 (fn1b) marker DOI:10.1016/j.ydbio.2013.08.012, DOI:10.15252/embr.202152901
7 Fibroblasts ENSDARG00000012405 (col1a1a) marker DOI:10.1172/JCI74783, DOI:10.15252/embr.202152901
8 Mesenchymal cells ENSDARG00000104340 (rspo1) marker DOI:10.1242/dev.068460, DOI:10.15252/embr.202152901
9 Macrophages ENSDARG00000044612 (c1qb) marker DOI:10.15252/embr.202152901
10 Neutrophils ENSDARG00000019521 (mpx) marker DOI:10.1189/jlb.4MR1114-524R, DOI:10.15252/embr.202152901
11 Coronary endothelial cells ENSDARG00000002644 (rgs5a) marker DOI:10.15252/embr.202152901
11 Coronary endothelial cells ENSDARG00000055100 (cxcl12b) marker DOI:10.15252/embr.202152901
13 Erythroid cells ENSDARG00000011166 (cahz) marker DOI:10.1182/bloodadvances.2018020156, DOI:10.15252/embr.202152901
13 Erythroid cells ENSDARG00000012881 (slc4a1a) marker DOI:10.1182/bloodadvances.2018020156, DOI:10.15252/embr.202152901
16 Thrombocytes ENSDARG00000074441 (gp1bb) marker DOI:10.15252/embr.202152901
17 Immune cells ENSDARG00000020788 (sla2) marker DOI:10.15252/embr.202152901

Cluster Cell type Gene id (symbol) Marker class Evidence
1 Macrophages ENSDARG00000090783 (mfap4) marker DOI:10.1371/journal.pone.0138949, DOI:10.15252/embr.202152901
1 Macrophages ENSDARG00000044612 (c1qb) marker DOI:10.15252/embr.202152901
1 Macrophages ENSDARG00000009087 (cd74a) marker DOI:10.15252/embr.202152901
2 Heart primordium ENSDARG00000019096 (myl7) marker DOI:10.15252/embr.202152901
3 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
3 Endocardial endothelial cells ENSDARG00000045141 (aqp8a.1) marker DOI:10.15252/embr.202152901
4 Fibroblasts ENSDARG00000036869 (tcf21) marker DOI:10.1242/dev.067041, DOI:10.1161/CIRCRESAHA.109.201400, DOI:10.15252/embr.202152901
4 Fibroblasts ENSDARG00000006526 (fn1b) marker DOI:10.1016/j.ydbio.2013.08.012, DOI:10.15252/embr.202152901
4 Fibroblasts ENSDARG00000012405 (col1a1a) marker DOI:10.1172/JCI74783, DOI:10.15252/embr.202152901
5 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
5 Endocardial endothelial cells ENSDARG00000045141 (aqp8a.1) marker DOI:10.15252/embr.202152901
6 Fibroblasts ENSDARG00000006526 (fn1b) marker DOI:10.1016/j.ydbio.2013.08.012, DOI:10.15252/embr.202152901
7 Mesenchymal cells ENSDARG00000027582 (angptl7) marker DOI:10.1186/s12862-016-0859-x, DOI:10.15252/embr.202152901
7 Mesenchymal cells ENSDARG00000104340 (rspo1) marker DOI:10.1242/dev.068460, DOI:10.15252/embr.202152901
7 Mesenchymal cells ENSDARG00000086189 (mgp) marker DOI:10.15252/embr.202152901
8 Immune cells ENSDARG00000057633 (cxcr4a) marker DOI:10.15252/embr.202152901
9 Coronary endothelial cells ENSDARG00000055100 (cxcl12b) marker DOI:10.15252/embr.202152901
9 Coronary endothelial cells ENSDARG00000014646 (aoc2) marker DOI:10.15252/embr.202152901
10 Fibroblasts ENSDARG00000006526 (fn1b) marker DOI:10.1016/j.ydbio.2013.08.012, DOI:10.15252/embr.202152901
11 Fibroblasts ENSDARG00000088116 (gstm.3) marker DOI:10.15252/embr.202152901
12 Erythroid cells ENSDARG00000011166 (cahz) marker DOI:10.1182/bloodadvances.2018020156, DOI:10.15252/embr.202152901
12 Erythroid cells ENSDARG00000012881 (slc4a1a) marker DOI:10.1038/ng1137, DOI:10.15252/embr.202152901

Cluster Cell type Gene id (symbol) Marker class Evidence
1 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
1 Endocardial endothelial cells ENSDARG00000045141 (aqp8a.1) marker DOI:10.15252/embr.202152901
2 Heart primordium ENSDARG00000019096 (myl7) marker DOI:10.15252/embr.202152901
3 Fibroblasts ENSDARG00000036869 (tcf21) marker DOI:10.1242/dev.067041, DOI:10.1161/CIRCRESAHA.109.201400, , DOI:10.15252/embr.202152901
3 Fibroblasts ENSDARG00000006526 (fn1b) marker DOI:10.1016/j.ydbio.2013.08.012, DOI:10.15252/embr.202152901
3 Fibroblasts ENSDARG00000012405 (col1a1a) marker DOI:10.1172/JCI74783, DOI:10.15252/embr.202152901
4 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
4 Endocardial endothelial cells ENSDARG00000045141 (aqp8a.1) marker DOI:10.15252/embr.202152901
5 Fibroblasts ENSDARG00000006526 (fn1b) marker DOI:10.1016/j.ydbio.2013.08.012, DOI:10.15252/embr.202152901
5 Fibroblasts ENSDARG00000012405 (col1a1a) marker DOI:10.1172/JCI74783, DOI:10.15252/embr.202152901
6 Macrophages ENSDARG00000090783 (mfap4) marker DOI:10.1371/journal.pone.0138949, DOI:10.15252/embr.202152901
6 Macrophages ENSDARG00000044612 (c1qb) marker DOI:10.15252/embr.202152901
6 Macrophages ENSDARG00000009087 (cd74a) marker DOI:10.15252/embr.202152901
7 Immune cells ENSDARG00000020788 (sla2) marker DOI:10.15252/embr.202152901
7 Immune cells ENSDARG00000105263 (ccl36.1) marker DOI:10.15252/embr.202152901
7 Immune cells ENSDARG00000041959 (cxcr4b) marker DOI:10.15252/embr.202152901
8 Mesenchymal cells ENSDARG00000027582 (angptl7) marker DOI:10.1186/s12862-016-0859-x, DOI:10.15252/embr.202152901
8 Mesenchymal cells ENSDARG00000104340 (rspo1) marker DOI:10.1242/dev.068460, DOI:10.15252/embr.202152901
8 Mesenchymal cells ENSDARG00000086189 (mgp) marker DOI:10.15252/embr.202152901
11 Erythroid cells ENSDARG00000011166 (cahz) marker DOI:10.1182/bloodadvances.2018020156, DOI:10.15252/embr.202152901
11 Erythroid cells ENSDARG00000012881 (slc4a1a) marker DOI:10.1038/ng1137, DOI:10.15252/embr.202152901

Cluster Cell type Gene id (symbol) Marker class Evidence
1 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
2 Endocardial endothelial cells ENSDARG00000045141 (aqp8a.1) marker DOI:10.15252/embr.202152901
3 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
5 Macrophages ENSDARG00000090783 (mfap4) marker DOI:10.1371/journal.pone.0138949, DOI:10.15252/embr.202152901
6 Fibroblasts ENSDARG00000036869 (tcf21) marker DOI:10.1242/dev.067041, DOI:10.1161/CIRCRESAHA.109.201400, DOI:10.15252/embr.202152901
6 Fibroblasts ENSDARG00000006526 (fn1b) marker DOI:10.1016/j.ydbio.2013.08.012, DOI:10.15252/embr.202152901
6 Fibroblasts ENSDARG00000012405 (col1a1a) marker DOI:10.1172/JCI74783, DOI:10.15252/embr.202152901
7 Macrophages ENSDARG00000044612 (c1qb) marker DOI:10.15252/embr.202152901
7 Macrophages ENSDARG00000009087 (cd74a) marker DOI:10.15252/embr.202152901
8 Coronary endothelial cells ENSDARG00000002644 (rgs5a) marker DOI:10.15252/embr.202152901
8 Coronary endothelial cells ENSDARG00000070038 (rbp2a) marker DOI:10.15252/embr.202152901
8 Coronary endothelial cells ENSDARG00000055100 (cxcl12b) marker DOI:10.15252/embr.202152901
9 Neutrophils ENSDARG00000057789 (lyz) marker DOI:10.1189/jlb.4MR1114-524R, DOI:10.15252/embr.202152901
9 Neutrophils ENSDARG00000019521 (mpx) marker DOI:10.1189/jlb.4MR1114-524R, DOI:10.15252/embr.202152901
10 Heart primordium ENSDARG00000019096 (myl7) marker DOI:10.15252/embr.202152901
11 Mesenchymal cells ENSDARG00000027582 (angptl7) marker DOI:10.1186/s12862-016-0859-x, DOI:10.15252/embr.202152901
11 Mesenchymal cells ENSDARG00000104340 (rspo1) marker DOI:10.1242/dev.068460, DOI:10.15252/embr.202152901
11 Mesenchymal cells ENSDARG00000086189 (mgp) marker DOI:10.15252/embr.202152901
12 Immune cells ENSDARG00000020788 (sla2) marker DOI:10.15252/embr.202152901
12 Immune cells ENSDARG00000057633 (cxcr4a) marker DOI:10.15252/embr.202152901
13 Thrombocytes ENSDARG00000018687 (itga2b) marker DOI:10.15252/embr.202152901
13 Thrombocytes ENSDARG00000074441 (gp1bb) marker DOI:10.15252/embr.202152901
14 Erythroid cells ENSDARG00000011166 (cahz) marker DOI:10.1182/bloodadvances.2018020156, DOI:10.15252/embr.202152901

Cluster Cell type Gene id (symbol) Marker class Evidence
1 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
1 Endocardial endothelial cells ENSDARG00000045141 (aqp8a.1) marker DOI:10.15252/embr.202152901
2 Macrophages ENSDARG00000090783 (mfap4) marker DOI:10.1371/journal.pone.0138949, DOI:10.15252/embr.202152901
2 Macrophages ENSDARG00000044612 (c1qb) marker DOI:10.15252/embr.202152901
2 Macrophages ENSDARG00000009087 (cd74a) marker DOI:10.15252/embr.202152901
3 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
3 Endocardial endothelial cells ENSDARG00000045141 (aqp8a.1) marker DOI:10.15252/embr.202152901
4 Fibroblasts ENSDARG00000006526 (fn1b) marker DOI:10.1016/j.ydbio.2013.08.012, DOI:10.15252/embr.202152901
4 Fibroblasts ENSDARG00000012405 (col1a1a) marker DOI:10.1172/JCI74783, DOI:10.15252/embr.202152901
5 Macrophages ENSDARG00000090783 (mfap4) marker DOI:10.1371/journal.pone.0138949, DOI:10.15252/embr.202152901
5 Macrophages ENSDARG00000044612 (c1qb) marker DOI:10.15252/embr.202152901
6 Mesenchymal cells ENSDARG00000027582 (angptl7) marker DOI:10.1186/s12862-016-0859-x, DOI:10.15252/embr.202152901
6 Mesenchymal cells ENSDARG00000104340 (rspo1) marker DOI:10.1242/dev.068460, DOI:10.15252/embr.202152901
6 Mesenchymal cells ENSDARG00000086189 (mgp) marker DOI:10.15252/embr.202152901
7 Heart primordium ENSDARG00000019096 (myl7) marker DOI:10.15252/embr.202152901
9 Macrophages ENSDARG00000044612 (c1qb) marker DOI:10.15252/embr.202152901
11 Neutrophils ENSDARG00000019521 (mpx) marker DOI:10.1189/jlb.4MR1114-524R, DOI:10.15252/embr.202152901
12 Coronary endothelial cells ENSDARG00000055100 (cxcl12b) marker DOI:10.15252/embr.202152901
14 Erythroid cells ENSDARG00000011166 (cahz) marker DOI:10.1182/bloodadvances.2018020156, DOI:10.15252/embr.202152901
14 Erythroid cells ENSDARG00000012881 (slc4a1a) marker DOI:10.1038/ng1137, DOI:10.15252/embr.202152901

Cluster Cell type Gene id (symbol) Marker class Evidence
1 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
1 Endocardial endothelial cells ENSDARG00000045141 (aqp8a.1) marker DOI:10.15252/embr.202152901
2 Macrophages ENSDARG00000090783 (mfap4) marker DOI:10.1371/journal.pone.0138949, DOI:10.15252/embr.202152901
2 Macrophages ENSDARG00000044612 (c1qb) marker DOI:10.15252/embr.202152901
2 Macrophages ENSDARG00000009087 (cd74a) marker DOI:10.15252/embr.202152901
3 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
3 Endocardial endothelial cells ENSDARG00000045141 (aqp8a.1) marker DOI:10.15252/embr.202152901
4 Fibroblasts ENSDARG00000006526 (fn1b) marker DOI:10.1016/j.ydbio.2013.08.012, DOI:10.15252/embr.202152901
4 Fibroblasts ENSDARG00000012405 (col1a1a) marker DOI:10.1172/JCI74783, DOI:10.15252/embr.202152901
5 Heart primordium ENSDARG00000019096 (myl7) marker DOI:10.15252/embr.202152901
6 Fibroblasts ENSDARG00000006526 (fn1b) marker DOI:10.1016/j.ydbio.2013.08.012, DOI:10.15252/embr.202152901
6 Fibroblasts ENSDARG00000012405 (col1a1a) marker DOI:10.1172/JCI74783, DOI:10.15252/embr.202152901
7 Mesenchymal cells ENSDARG00000027582 (angptl7) marker DOI:10.1186/s12862-016-0859-x, DOI:10.15252/embr.202152901
7 Mesenchymal cells ENSDARG00000104340 (rspo1) marker DOI:10.1242/dev.068460, DOI:10.15252/embr.202152901
7 Mesenchymal cells ENSDARG00000086189 (mgp) marker DOI:10.15252/embr.202152901
8 Erythroid cells ENSDARG00000011166 (cahz) marker DOI:10.1182/bloodadvances.2018020156, DOI:10.15252/embr.202152901
8 Erythroid cells ENSDARG00000012881 (slc4a1a) marker DOI:10.1038/ng1137, DOI:10.15252/embr.202152901
9 Immune cells ENSDARG00000020788 (sla2) marker DOI:10.15252/embr.202152901
9 Immune cells ENSDARG00000057633 (cxcr4a) marker DOI:10.15252/embr.202152901
10 Coronary endothelial cells ENSDARG00000055100 (cxcl12b) marker DOI:10.15252/embr.202152901
14 Erythroid cells ENSDARG00000011166 (cahz) marker DOI:10.1182/bloodadvances.2018020156, DOI:10.15252/embr.202152901
14 Erythroid cells ENSDARG00000012881 (slc4a1a) marker DOI:10.1038/ng1137, DOI:10.15252/embr.202152901
15 Neutrophils ENSDARG00000057789 (lyz) marker DOI:10.1189/jlb.4MR1114-524R, DOI:10.15252/embr.202152901
15 Neutrophils ENSDARG00000019521 (mpx) marker DOI:10.1189/jlb.4MR1114-524R, DOI:10.15252/embr.202152901
16 Coronary endothelial cells ENSDARG00000002644 (rgs5a) marker DOI:10.15252/embr.202152901

Cluster Cell type Gene id (symbol) Marker class Evidence
1 Fibroblasts ENSDARG00000006526 (fn1b) marker DOI:10.1016/j.ydbio.2013.08.012, DOI:10.15252/embr.202152901
1 Fibroblasts ENSDARG00000012405 (col1a1a) marker DOI:10.1172/JCI74783, DOI:10.15252/embr.202152901
2 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
2 Endocardial endothelial cells ENSDARG00000045141 (aqp8a.1) marker DOI:10.15252/embr.202152901
3 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
3 Endocardial endothelial cells ENSDARG00000045141 (aqp8a.1) marker DOI:10.15252/embr.202152901
4 Heart primordium ENSDARG00000019096 (myl7) marker DOI:10.15252/embr.202152901
5 Endocardial endothelial cells ENSDARG00000070266 (spock3) marker DOI:10.15252/embr.202152901
6 Macrophages ENSDARG00000090783 (mfap4) marker DOI:10.1371/journal.pone.0138949, DOI:10.15252/embr.202152901
6 Macrophages ENSDARG00000044612 (c1qb) marker DOI:10.15252/embr.202152901
6 Macrophages ENSDARG00000009087 (cd74a) marker DOI:10.15252/embr.202152901
8 Mesenchymal cells ENSDARG00000027582 (angptl7) marker DOI:10.1186/s12862-016-0859-x, DOI:10.15252/embr.202152901
8 Mesenchymal cells ENSDARG00000104340 (rspo1) marker DOI:10.1242/dev.068460, DOI:10.15252/embr.202152901
8 Mesenchymal cells ENSDARG00000086189 (mgp) marker DOI:10.15252/embr.202152901
9 Immune cells ENSDARG00000057633 (cxcr4a) marker DOI:10.15252/embr.202152901
10 Coronary endothelial cells ENSDARG00000055100 (cxcl12b) marker DOI:10.15252/embr.202152901
12 Fibroblasts ENSDARG00000088116 (gstm.3) marker DOI:10.15252/embr.202152901
13 Erythroid cells ENSDARG00000011166 (cahz) marker DOI:10.1182/bloodadvances.2018020156, DOI:10.15252/embr.202152901
13 Erythroid cells ENSDARG00000012881 (slc4a1a) marker DOI:10.1038/ng1137, DOI:10.15252/embr.202152901
14 Thrombocytes ENSDARG00000018687 (itga2b) marker DOI:10.15252/embr.202152901
14 Thrombocytes ENSDARG00000074441 (gp1bb) marker DOI:10.15252/embr.202152901
15 Neutrophils ENSDARG00000057789 (lyz) marker DOI:10.1189/jlb.4MR1114-524R, DOI:10.15252/embr.202152901
15 Neutrophils ENSDARG00000019521 (mpx) marker DOI:10.1189/jlb.4MR1114-524R, DOI:10.15252/embr.202152901